a Record control number 000312048500005

a ISSN 0972-9062

a Original cataloging agency NEU GrandLibrary

a Language code of text/sound track or separate title eng

a Classification number WC 39

b Item number .E93 2012

a Personal name Oluwasogo, Olalubi Adewole.

h Medium Electronic Document

a Title Evaluation of host humoral antibody production against Plasmodium falciparum recombinant circumsporozoite antigen in Nigerian children

c Statement of responsibility, etc Olalubi Adewole Oluwasogo, Ogunlana Oluseyi Ebenezer, Anumudu Chiaka.

c Date of publication, distribution, etc 2012.

b Name of publisher, distributor, etc Malarıa Research Centre

a Summary, etc Background & objectives: The challenge of malaria and efforts targeted at developing malaria vaccines triggered this study on the reactivity of IgG and its subclasses in the test serum specific to CSP. This work was directed at assessing the influence of age and gender on host humoral antibody against Plasmodium falciparum recombinant circumsporozoite antigen in Nigerian children. Methods: In all, 67 serum samples (> 10,000 parasites/mu l of blood) collected from malaria-infected children at the University College Hospital, Ibadan during the transmission season were analyzed by ELISA. Results: The mean absorbance values of IgG subclasses reactive against P. falciparum CSP appeared to be age-dependent and ranged from 0.01 for IgG4 in younger children to 0.95 for IgG3 in older children. The sixty-seven subjects investigated in this study had significantly higher mean IgG1 and IgG3 than the uninfected controls (p < 0.01). This follows the order IgG3 > IgG1 > IgG2 > IgG4 which confirmed the prevalence of the cytophilic antibodies (IgG1 and IgG3) in 65% of the malaria infected children over the non-cytophilic subclasses (IgG2 and IgG4). Similarly, there was low production of IgG4 and IgG2 levels in 35% of the subjects compared with control. IgG was detected in the serum of North American Subjects (NAS) which served as negative control for CSP-specific IgG subclasses. Although the NAS titre was lower than that of the malaria subjects in Nigeria, its IgG2 was, however, higher (0.16) than that of other subclasses. The mean absorbance values of total serum IgG subclass were higher than those of IgG subclasses specific to P. falciparum circumsporozoite antigen. The mean absorbance values of the total serum IgG subclass follows the order IgG2 > IgG1 > IgG4 > IgG3. Interpretation & conclusion: Age and gender-dependent correlations of results suggest that acquired immunity could play a significant role in protection from malaria. Antibody levels are higher in male than female children of the same age group. Antibody levels also increase with age in both the male and female children.

a Topical term or geographic name as entry element Infectious Diseases

a Topical term or geographic name as entry element Near East University Article

a Topical term or geographic name as entry element Yakın Doğu Üniversitesi Makale

a Topical term or geographic name as entry element Parasitology

a Topical term or geographic name as entry element Cytophilic antibodies (IgG1 and IgG3)

a Topical term or geographic name as entry element Plasmodium falciparum circumsporozoite protein (PfCSP)

g Relationship information SEP 2012, Vol. 49 Issue 3, p151-156

x International Standard Serial Number 09729062

t Title Journal Of Vector Borne Dıseases