- The effects of cold pretreatments on pepper (Capsicum annuum L.) buds to the relationship between the endogenous abscisic acid (ABA) content of anthers and in vitro androgenesis were investigated. Cold shock treatments at +4degreesC at periods of 48 and 96 hours were used. The anthers were cultured in the MS medium with 4 mg/l NAA and 1 mg/l BA. Activated charcoal was added to the medium at a concentration of 0.25%. The cultures were incubated at +29degreesC under conditions of continuous illumination. The ABA analyses were made at different stages, (1) after the buds were separated from the plants (control), (2) after the cold treatments, and (3) at the 50th day of the incubation. The ABA amount of the anthers that had cold treatment always remained lower than the control anthers. However, the most embryo formation was obtained from the control anthers not subjected to cold treatments (12.5%). These results indicated that the ABA content in anthers is not the only factor for obtaining embryos from the anther culture of peppers.
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