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022 _a0197-0186
040 _aNEU
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050 0 4 _aWL 140
100 1 _9573261
_aYildirim, Fatos Belgin.
245 1 0 _aMechanism of the beneficial effect of melatonin in experimental Parkinson's disease
_cFatos Belgin Yildirim, Ozlem Ozsoy, Gamze Tanriover, Yasemin Kaya, Eren Ogut, Burcu Gemici, Sayra Dilmac, Ayse Ozkan, Aysel Agar, Mutay Aslan.
260 _aPergamon-Elsevier Science Ltd.,
_c2014.
520 _aThis study aimed to elucidate locomotor activity changes in 6-hydroxydopamine (6-OHDA) induced Parkinson's disease (PD) and investigate the possible beneficial effects of melatonin on altered levels of locomotor activity, cyclooxygenase (COX), prostaglandin E2 (PGE2), nuclear factor kappa-B (NF-kappa B), nitrate/nitrite and apoptosis. Male Wistar rats were divided into five groups: vehicle (V), melatonin-treated (M), 6-OHDA-injected (6-OHDA), 6-OHDA-injected + melatonin-treated (6-OHDA-Mel) and melatonin treated + 6-OHDA-injected (Mel-6-OHDA). Melatonin was administered intraperitoneally at a dose of 10 mg/kg/day for 30 days in M and Mel-6-OHDA groups, for 7 days in 6-OHDA-Mel group. Experimental PD was created stereotactically via unilateral infusion of 6-OHDA into the medial forebrain bundle (MFB). The 6-OHDA-Mel group started receiving melatonin when experimental PD was created and treatment was continued for 7 days (post-treatment). In the Mel-6-OHDA group, experimental PD was created on the 23rd day of melatonin treatment and continued for the remaining 7 days (pre- and post-treatment). Locomotor activity performance decreased in 6-OHDA group compared with vehicle; however melatonin treatment did not improve this impairment. Nuclear factor kappa Bp65 and Bcl-2 levels were significantly decreased while COX, PGE2 and caspase-3 activity were significantly increased in 6-OHDA group. Melatonin treatment significantly decreased COX, PGE2 and caspase-3 activity, increased Bcl-2 and had no effect on NE-kappa B levels in experimental PD. 6-Hydroxydopamine injection caused an obvious reduction in TH positive dopaminergic neuron viability as determined by immunohistochemistry. Melatonin supplementation decreased dopaminergic neuron death in 6-OHDA-Mel and Mel-6-OHDA groups compared with 6-OHDA group. Melatonin also protected against 6-OHDA-induced apoptosis, as identified by increment in Bcl-2 levels in dopaminergic neurons. The protective effect of melatonin was more prominent for most parameter following 30 days treatment (pre- and post-) than 7 days post-treatment. In summary, melatonin treatment decreased dopaminergic neuron death in experimental PD model by increasing Bcl-2 protein level and decreasing caspase-3 activity. (C) 2014 Elsevier Ltd. All rights reserved.
650 0 _9124265
_aNeurology
650 0 _9572720
_aNear East University Article
650 0 _9572723
_aYakın Doğu Üniversitesi Makale
650 0 _9573262
_aMelatonin
650 0 _9573263
_a6-Hydroxydopamine
650 0 _9573264
_aCyclooxygenase
650 0 _9573265
_a Nf-Kappa-B
773 _gDEC 2014 , Vol 79, p1-11
_x01970186
_tNeurochemistry International
856 _uhttp://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.1016/j.neuint.2014.09.005
942 _x1000007
_kWL0000140M432014
_cOED
999 _c241345